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Image Search Results
Journal: bioRxiv
Article Title: Grb7 knockout mice develop normally but litters born to knockout females fail to thrive
doi: 10.1101/2023.06.29.546912
Figure Lengend Snippet: A . Allele descriptions, primer locations (arrowheads), and breeding schema. The wild-type Grb7 allele is shown for reference (top), with variations below. Mice with the “knockout-first” lacZ gene-trap allele ( Grb7 lacZ ) were bred to mice carrying an actin-driven FLP recombinase allele, resulting in excision of the lacZ cassette, yielding a conditional knockout allele, Grb7 FL . A subsequent cross with mice expressing CMV-driven cre recombinase removed Grb7 exons 2-7, yielding the knockout allele ( Grb7 del ). B . PCR verification from tail biopsy DNA of stepwise generation of each allele: common primer set for transgenic allele (T+/T-), Grb7 lacZ allele (A+/A-), Grb7 FL (A+/B-) and Grb7 - (A+/C-primers). C . Western blotting of total liver lysate from Grb7 +/+ , Grb7 +/lacZ , and Grb7 lacZ/lacZ mice for Grb7 and β-galactosidase.
Article Snippet: Cre-deleter mice expressing ubiquitous CMV-driven
Techniques: Knock-Out, Expressing, Transgenic Assay, Western Blot